Semester: 6 / Spring
Lectures/Classes: 45 / 30 hours
Field of study: Biotechnology
Study cycle: 1st cycle
Type of course: compulsory
Prerequisites: biology, biochemistry
Contact person: dr Adam Dobrowolski;
Short description: During the course students acquire detailed knowledge of cellular macromolecules structure and their functions. They learn instrumental and bioinformatique methods of genome, transcryptom, proteome and metabolome studies. They perform restriction analyses of plasmid, genes or DNA fragments and plan starters and conditions for PCR. Students known the gene expression regulation in Prokaryota, Archea and Eukaryota as well as postranscriptomic and posttranslational modification.
Full description: Macromolecules: structure characteristics, methods of analysis Genome: organization of genetic material, gene structure and regulation of expression , noncoding DNA. DNA sequencing. Transcriptome: different RNAs, function, importance of RNA in gene expression and protein biosynthesis (ribosome, tRNA, rRNA, hnRNA, splicing and modification of RNA, ribozyme, riboswitches, RNAi ). Proteome: methods of analysis (2D electrophoresis andMaldi-TOF), function, biosynthesis, posttranslational modification, protein degradation Metabolic molecular biology: replication of DNA and reparation processes, transcription of DNA, translation and gene expression regulation in Prokaryota, Archeaand Eukaryota. Internet: genomic and proteomic data bases, bioinformatics’ tools.
Bibliography: Compulsory: 1. Biologia molekularna, Krótkie wykłady, Turner i in., PWN, Warszawa, 2000; 2. Genomy, Brown T., PWN, Warszawa, 2001; 3. Genomy, Brown T., PWN, Warszawa, 2009 (nowe zmienione wydanie); 4. Zasoby Internetu (NCBI, PDB, SGD i inne) Supplementary: 1. Genes VIII, Lewin B., Pearson Prentice Hall, NY, 2004. 2. Biofizyka kwasów nukleinowych dla biologów, Bryszewska i Leyko , PWN,Warszawa, 2000; 3. Zasady analizy genomu, Primrose S.B., WNT, Warszawa, 1999; 4. Podstawy bioinformatyki Xiomg J.,WUW, 2009
Learning outcomes: After completing the course student In terms of knowledge: 1. has the knowledge of the structure of important macromolecules, genome organization gene expression regulation with respect to differences between virus, Prokaryote, Eukaryote and Archaea organisms, 2. is able to define the notion of genome, transcriptome, proteome and metabolome, 3. knows the methods of gene amplification in vitro ( PCR, Real Time-PCR, RT-PCR) and restriction analysis, 4. Student has the ability to describe importance of the recent results in genome sequencing for new medicine researches, therapeutic strategy (gene silencing, gene therapy, personalized therapy) and microorganisms improvement to better or new application in biotechnological processes. In terms of skills: 1.understands molecular and cellular basis of organism function, 2. has skills to work with basic apparatus used in genome and proteome analyze, 3. knows basic and experimental methods used in molecular analysis, understands restriction analysis and has the ability to use genomic and proteomic database and basic bioinformatics tools, 4. knows how defined starters and condition to PCR amplification of desired DNA fragment. In terms of social competences: 1. is aware of the rules required when working with genetic material and the molecular biology lab, 2. is aware of the importance of professional and ethical responsibility for the social consequences of the use of the tools of molecular biology and genetic engineering, 3. is able to team-work (taking different roles, shows willingness to cooperate is aware of the responsibility of the team for the results of the experiment, 4. understands the need to learn lifelong and update knowledge related to their profession.
Assessment methods and assessment criteria: Grade from laboratorz classes 50%, Exam 50%

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