Course title: ENZYMOLOGY
Semester: 6 / Spring
Lectures/Classes: 15 / 30 hours
Field of study: Biotechnology
Study cycle: 1st cycle
Type of course: compulsory
Prerequisites: organic chemistry, biochemistry, general microbiology
Contact person: dr inż. Wojciech Łaba; wojciech.laba@upwr.edu.pl
Short description: The course aims to present the structure and properties of enzymes, kinetics of enzymatic reactions and methods of enzymes purification and preservation. During the classes students carry out experiments consisting of enzymes purification, determination of their kinetic parameters and analysis of the influence of inhibitors on their enzymatic activity. The classes allow the students to acquire skills for analyzing enzymatic activity as well as to calculate the protein purification balance.
Full description: Kinetics, enzyme properties, mechanisms of enzymatic catalysis, rules of isolation of enzymatic proteins from plants anminals and microorganisms, purification techniques involving fractionated precipitation, specific adsorption, ion exchange chromatography, affinity chromatography, electrophoretic techniques, immobilization of proteins as a method for their stabilization, evaluation criteria for purity of enzymatic preparations.
Bibliography: Compulsory: 1. The enzyme reference, Purich D.,L., Allison R.,D., Acad Press, USA, 2002, 2. Introduction to Biotechnology, Thieman W.,J., Palladino M.,A., Pearson Education, 2004, 3. Practical enzymology, Bisswanger H., Wileh-VCh, 2004, 4. Enzyme biocatalysis, Principles and applications, Ed. Illanes A., Springer Sci, 2008, 5. Fundamentals of enzymology, Price N.C., Stevens L., Oxford Univ.press, 2009, 6. Basic methods for the Biochemical Lab, Holtzhauer M., Springer, 2006.
Learning outcomes: After completing the course student In terms of knowledge: 1. Knows mode of action of enzymes, understands mechanisms of enzymatic catalysis, 2. Knows possible applications of enzymes in various biotechnological processes, 3. Knows methods of enzymes isolation from plant, animal and microbial sources, 4. Knows modern techniques for purification of enzymatic proteins and methods for evaluation of purity of enzymatic preparations. In terms of skills: 1. Understands the contribution of enzymes in metabolism of organisms at various levels of development, 2. Selects proper techniques for purification of enzymes, based on their properties, 3. Applies proper methods of enzymatic activity determination and calculates efficiency of the purification process, 4. Is capable of selecting proper enzyme for a specific biotechnological process. In terms of social competences: 1. Is aware of the responsibility for the tasks resolved in a work-group and for the laboratory equipment, 2. Understands the importance of respecting the rules of industrial safety.
Assessment methods and assessment criteria: arithmetic mean from laboratory classes and final written exam

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